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Neurological — Sleep✓ FDA Approved

DSIP

Also known as: Delta Sleep-Inducing Peptide · Delta Sleep Peptide

MW

848.82 Da

Amino Acids

9 AA

Half-Life

7-8 minutes (rapid)

Route

SubQ, IV

CAS

62568-57-4

Formula

C35H48N10O15

Amino Acid Sequence

WAGGDASGE

Mechanism of Action

DSIP (Delta Sleep-Inducing Peptide) is a nonapeptide (9 amino acids: Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) originally isolated from rabbit brain during induced sleep. It modulates sleep architecture, particularly slow-wave (delta) sleep.

SLEEP MODULATION: Acts on multiple neuromodulatory systems rather than a single receptor. Modulates GABA-A receptor complex sensitivity, serotonergic tone, and delta opioid receptor systems. Promotes entry into and maintenance of slow-wave (Stage 3/4) sleep — the most restorative sleep phase where GH secretion peaks, memory consolidation occurs, and tissue repair is maximized.

STRESS AXIS: Reduces cortisol and normalizes the hypothalamic-pituitary-adrenal (HPA) axis stress response. Some evidence for anti-stress and adaptogenic effects.

LH MODULATION: Modulates LH (luteinizing hormone) secretion, suggesting hypothalamic interaction.

The exact primary receptor/mechanism remains debated after 40+ years of research — DSIP likely acts as a neuromodulatory peptide affecting multiple systems simultaneously rather than through a single classical receptor-ligand interaction.

Dosing Protocol

Low Dose

███ – ███ mcg/day

Standard Dose

███ mcg/day

High Dose

███ – ███ mcg/day

Dosing protocols are for paid members

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Frequency

Once nightly before bed.

Half-Life

7-8 minutes (rapid)

Reconstitution Guide

Full reconstitution protocol with BAC water volumes, concentration math, and units-to-draw per dose is available on the Clinical plan.

Unlock reconstitution guide →

Clinical Warnings

Mechanism not fully understood after 40+ years.

Short half-life (~15 min) limits practical efficacy.

Mixed results in clinical studies — reproducibility issues.

Quality concerns from gray-market sources.

Not FDA approved.

Contraindications

Absolute

Pregnancy

Relative Cautions

Epilepsy

Depression

Sleep apnea

Side Effect Profile

Mild

  • Morning grogginess
  • Mild headache

Moderate

  • Oversleeping
  • Vivid dreams
  • Transient hypotension

Synergistic Peptides

EpithalonSelankCJC-1295

Common Stacks

Epithalon

Research Status

LIMITED. PMID 6324896 (Schoenenberger 1978): Original DSIP isolation and sleep-promoting activity characterization. PMID 6305877 (Müller 1984): Human REM and slow-wave sleep effects. Mixed reproducibility in clinical studies. Mechanism still debated.

Frequently Asked Questions

How does DSIP work?

DSIP (Delta Sleep-Inducing Peptide) is a nonapeptide (9 amino acids: Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) originally isolated from rabbit brain during induced sleep. It modulates sleep architecture, particularly slow-wave (delta) sleep. SLEEP MODULATION: Acts on multiple neuromodulatory systems rather than a single receptor. Modulates GABA-A receptor complex sensitivity, serotonergic tone, and delta opioid receptor systems. Promotes entry into and maintenance of slow-wave (Stage 3/4) sleep — th

What is the standard dose of DSIP?

DSIP dosing protocols are available with a ClinPep Clinical subscription. Dosing varies by indication and patient factors — consult a licensed healthcare provider. General frequency: Once nightly before bed.

What is the half-life of DSIP?

The half-life of DSIP is 7-8 minutes (rapid). This determines optimal dosing frequency and timing.

Who should not use DSIP?

DSIP is absolutely contraindicated in: Pregnancy. Use with caution in: Epilepsy; Depression; Sleep apnea.

What are the side effects of DSIP?

Common mild side effects include: Morning grogginess, Mild headache. Moderate effects: Oversleeping, Vivid dreams, Transient hypotension.

What peptides stack well with DSIP?

DSIP is commonly stacked with: Epithalon, Selank, CJC-1295.

How do you reconstitute DSIP?

DSIP is reconstituted with bacteriostatic water. Exact volumes, concentrations, and units-to-draw calculations are available in the ClinPep Clinical plan. Always follow your compounding pharmacy's instructions.

How long should you cycle DSIP?

DSIP cycle protocols vary by indication. Detailed cycle length, on/off schedules, and monitoring guidelines are available with ClinPep Clinical access. Consult your healthcare provider for personalized cycling guidance.

References & Citations

4 PubMed studies · 3 clinical trials

Pichia pastoris secreted peptides crossing the blood-brain barrier and DSIP fusion peptide efficacy in PCPA-induced insomnia mouse models.

Mu Xiaoxiao, Qu Lijun, Yin Liquan, Wang Libo et al.. Frontiers in pharmacology. 2024

PubMed: 39444618DOI ↗C — Research Article

Pichia pastoris-secreted delta sleep inducing peptide and crossing the blood-brain barrier peptides (DSIP-CBBBP) fusion peptides holds significant promise for its potential sleep-enhancing and neurotr

Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke.

Tukhovskaya Elena A, Ismailova Alina M, Shaykhutdinova Elvira R, Slashcheva Gulsara A et al.. Molecules (Basel, Switzerland). 2021

PubMed: 34500605DOI ↗C — Research Article

Background and Objectives: Mutual effect of the preliminary and therapeutic intranasal treatment of SD rats with DSIP (8 days) on the outcome of focal stroke, induced with intraluminal middle cerebral

DSIP-Like KND Peptide Reduces Brain Infarction in C57Bl/6 and Reduces Myocardial Infarction in SD Rats When Administered during Reperfusion.

Tukhovskaya Elena A, Shaykhutdinova Elvira R, Ismailova Alina M, Slashcheva Gulsara A et al.. Biomedicines. 2021

PubMed: 33918965DOI ↗C — Research Article

A structural analogue of the DSIP, peptide KND, previously showed higher detoxification efficacy upon administration of the cytotoxic drug cisplatin, compared to DSIP. DSIP and KND were investigated u

Phosphorylated delta sleep inducing peptide restores spatial memory and p-CREB expression by improving sleep architecture at high altitude.

Roy Koustav, Chauhan Garima, Kumari Punita, Wadhwa Meetu et al.. Life sciences. 2018

PubMed: 30107169DOI ↗C — Research Article

Sleep loss at high altitude (HA) play major role in worsening of neuropsychological functions, such as attention, memory and decision making. This study investigates the role of phosphorylated delta s

Registered Clinical Trials

Pregabalin in CIPN

NCT02394951COMPLETEDNA

Impact of Hot Flashes on Sleep and Mood Disturbance

NCT01116401COMPLETEDPHASE4

Botulinum Toxin in the Palatopharyngeal Muscle for the Treatment of Obstructive Sleep Apnea

NCT07399782NOT_YET_RECRUITINGPHASE2

Symptom Indications

InsomniaPoor sleep qualityChronic stressCortisol dysregulationNight sweats

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This information is for educational and research reference purposes only. ClinPep does not provide medical advice, diagnosis, or treatment recommendations. All protocols should be reviewed by a licensed healthcare provider.